mouse monoclonal wt 1  (Santa Cruz Biotechnology)

Journal: Genes & Diseases

Article Title: Establishment and functional characterization of the reversibly immortalized mouse glomerular podocytes (imPODs)

doi: 10.1016/j.gendis.2018.04.003

Figure Lengend Snippet: The imPOD cells express podocyte markers . The imPOD and parental tsPC (at 33 °C + 100U/ml γ-interferon) cells were seeded at subconfluence and stained with antibodies against the podocyte specific marker (WT-1) and Synaptopodin ( A ), as well as the slit diaphragm complex related markers (Nephrin, Tubulin and Vinculin) ( B ). Stains without primary antibodies were used as negative controls. Cell nuclei were counter-stained with DAPI. Representative images are shown.

Article Snippet: Immunofluorescence staining was performed as described., , , , Briefly, subconfluent imPOD or tsPC cells were seeded in 24-well plates overnight, fixed with 4% paraformaldehyde, permeabilized with 1% NP-40, and blocked with 10% donkey serum (Jackson Immuno-Research Laboratories, West Grove, PA, USA), followed by incubating with the WT-1, Synaptopodin, Nephrin, Tubulin or Vinculin mouse monoclonal antibody (Santa Cruz Biotechnology, Dallas, TX, USA) for 2 hrs at room temperature., , After being washed, cells were incubated with FITC labeled anti-mouse IgG secondary antibody (Jackson ImmunoResearch Laboratories, West Grove, PA) for 30 min.

Techniques: Staining, Marker